三种多酚类化合物对模拟间断性高原低氧暴露小鼠肠道菌群的影响*

doi:10.12047/j.cjap.6241.2022.073

中国应用生理学杂志 ›› 2022, Vol. 38 ›› Issue (5): 392-396.doi: 10.12047/j.cjap.6241.2022.073

三种多酚类化合物对模拟间断性高原低氧暴露小鼠肠道菌群的影响^*

潘存瑶^1,2,3+, 张宝怡¹⁺, 梁兰兰²⁺, 刘辉², 郭长江¹, 陈照立¹, 王新兴^1△

1.军事科学院军事医学研究院环境医学与作业医学研究所, 天津 300050;
2.兰州大学公共卫生学院, 甘肃兰州 730000;
3.成都市疾病预防控制中心, 四川成都 610000

收稿日期:2021-12-13 修回日期:2022-09-28 出版日期:2022-09-28 发布日期:2023-04-23
通讯作者: ^△Tel: +86-22-84655206; E-mail: wxxemail@sina.cn.
作者简介:⁺:为共同第一作者
基金资助:
^*国家自然科学基金面上项目(31971106,18CXZ044)

Effects of three Polyphenolic compounds on the intestinal flora of mice exposed simulated intermittent plateau hypoxia

PAN Cun-yao^1,2,3+, ZHANG Bao-yi¹⁺, LIANG Lan-lan²⁺, LIU Hui², GUO Chang-jiang¹, CHEN Zhao-li¹, WANG Xin-xing^1△

1. Institute of Environmental and Operational Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Tianjin 300050;
2. School of Public Health, Lanzhou University, Lanzhou 730000;
3. Chengdu Center for Disease Control and Prevention, Chengdu 610000, China

Received:2021-12-13 Revised:2022-09-28 Online:2022-09-28 Published:2023-04-23

摘要/Abstract

摘要： 目的: 探索三种多酚类化合物对高原低氧暴露小鼠肠道菌群的保护作用。方法: 将60只雄性Balb/c小鼠随机分为平原对照组、模拟高原低氧组、高原原花青素组、高原槲皮素组、高原白藜芦醇组,每组12只。原花青素、槲皮素、白藜芦醇分别以50 mg/kg ,100 mg/kg,20 mg/kg的剂量进行灌胃,三种药物干预且高原模拟舱模拟海拔6 000 m暴露30 d后,收集血清进行二胺氧化酶(DAO)检测,收集小鼠无菌粪便,回肠组织固定并HE染色,16S rRNA技术检测小鼠肠道菌群多样性以及菌群组成。 结果: HE染色结果显示,模拟高原低氧组较平原对照组回肠组织损伤明显,血清DAO浓度显著升高(P＜0.05),但肠道菌群物种丰度、菌群多样性差异无统计学意义。与模拟高原低氧组相比,高原槲皮素、白藜芦醇组小鼠回肠组织结构得以改善,血清DAO水平降低,且肠道菌群物种丰度、α多样性降低(P＜0.05),拟杆菌相对丰度降低(P＜0.05),厚壁菌升高(P＜0.05),谷氨酸棒状杆菌、罗伊氏乳杆菌相对丰度升高(P＜0.05)。结论: 槲皮素和白藜芦醇可有效增加小鼠有益菌群的丰度,抑制并降低条件致病菌,对高原低氧暴露小鼠肠道有一定的保护作用。

关键词: 高原低氧, 原花青素, 槲皮素, 白藜芦醇, 肠道菌群

Abstract: Objective: To investigate the protective effects of three Polyphenolic compounds on intestinal microbial communities in mice exposed intermittent plateau hypoxia. Methods: In this study, 60 healthy male Balb/c mice were randomly divided into plain control group, plateau control group, primary anthocyanin intervention group, quercetin intervention group and resveratrol intervention group, 12 mice in each group. Primary anthocyanin, quercetin and resveratrol were administrated by gavage at the doses of 50, 100 and 20 mg/kg in pharmacological intervention group, respectively. After exposure of the mice to simulation plateau-condition for 30 days, the serum samples were collected for DAO testing, sterile feces were collected in mice, and the diversity and genus level of the mouse gut bacteria were detected by using 16S rRNA technology. Ileum tissue was fixed and stained with HE. Results: HE staining showed that the plateau control group had significant damage to the intestinal tissue structure compared to the plain control group, and the serum DAO concentration was increased (P＜0.05), but there was no statistical difference in the abundance and diversity of intestinal flora species. Contrast to simulated intermittent plateau hypoxia group, the structure of the intestine tissue and the level of DAO in the quercetin intervention group and resveratrol intervention group were improved(P＜0.05), the abundance and α diversity of the intestinal flora were decreased, the relative abundance of Bacteroidetes was reduced(P＜0.05), and the Firmicutes was increased. Concomitantly, significant decreases in relative abundance were observed for Corynebacterium glutamicum and Lactobacillus reuteri(P＜ 0.05). Conclusion: Quercetin and resveratrol showed some degree of protection to mice intestinal microbial communities, and increased the diversity and the abundance of the dominant flora and inhibited the growth of conditional pathogenic bacteria.

Key words: plateau hypoxia, Procyanidin, Quercetin, Resveratrol, intestinal microbial

中图分类号:

R73-3

潘存瑶, 张宝怡, 梁兰兰, 刘辉, 郭长江, 陈照立, 王新兴. 三种多酚类化合物对模拟间断性高原低氧暴露小鼠肠道菌群的影响^*[J]. 中国应用生理学杂志, 2022, 38(5): 392-396.

PAN Cun-yao, ZHANG Bao-yi, LIANG Lan-lan, LIU Hui, GUO Chang-jiang, CHEN Zhao-li, WANG Xin-xing. Effects of three Polyphenolic compounds on the intestinal flora of mice exposed simulated intermittent plateau hypoxia[J]. CJAP, 2022, 38(5): 392-396.

参考文献

[1] Hogberg N, Carlsson PO, Hillered L, et al. Intraluminal intestinal microdialysis detects markers of hypoxia and cell damage in experimental necrotizing enterocolitis[J]. J Pediatr Surg, 2012, 47(9): 1646-1651.
[2] Liu H, Li W, Wang X, et al. Early gut mucosal dysfunction in patients with acute pancreatitis [J]. Pancreas, 2008, 36(2): 192-196.
[3] Bonkowsky JL, Son JH. Hypoxia and connectivity in the developing vertebrate nervous system [J]. Dis Model Mech, 2018, 11(12): 150-154.
[4] Simon MC, Liu L, Barnhart BC, et al. Hypoxia-induced signaling in the cardiovascular system [J]. Annu Rev Physiol, 2008, 70: 51-71.
[5] Gao Z, Wu H, Zhang K, et al. Protective effects of grape seed procyanidin extract on intestinal barrier dysfunction induced by a long-term high-fat diet[J]. J Funct Foods, 2020, 64: 3-7.
[6] 陈樱萌, 胡满江, 曾钰鹏, 等. 槲皮素对肠道菌群的调节作用研究[J]. 食品研究与开发, 2020, 41(10): 6-15.
[7] Zhu F, Zheng J, Xu F, et al. Resveratrol alleviates dextran sulfate sodium-induced acute ulcerative colitis in mice by mediating PI3K/Akt/VEGFA pathway[J]. Front Pharmacol, 2021, 12: 693982.
[8] Guarner F. Role of intestinal flora in health and disease[J]. Nutr Hosp, 2007, 22: 14-19.
[9] Nord CE. The effect of antimicrobial agents on the ecology of the human intestinal microflora[J]. Vet Microbiol, 1993, 35(3-4): 193-197.
[10] Parfenov AI, Bondarenko VM. What we gained from a century of investigations of symbiontic intestinal microflora[J]. Terapevt Arkh, 2012, 84(2): 5-10.
[11] Gao H, Chi X, Li G, et al. Gut microbial diversity and stabilizing functions enhance the plateau adaptability of Tibetan wild ass (Equus kiang)[J]. Microbiologyopen, 2020, 9(6): 10-13.
[12] Zhang J, Zhang J, Wang R, et al. Effects of gut microbiota on drug metabolism and guidance for rational drug use under hypoxic conditions at high altitudes[J]. Curr Drug Metab, 2019, 20(2): 155-165.
[13] 伍荷洁, 张丽萍, 舒青龙. 不同浓度理中汤多糖作为碳源对肠道拟杆菌及厚壁菌体外生长的影响[J]. 山东医药, 2018, 58(23): 29-32.
[14] 张晓梅, 高宇洁, 杨玲, 等. 谷氨酸棒杆菌中氨基酸分泌转运蛋白及其代谢改造研究进展[J]. 生物工程学报, 2020, 36(11): 2250-2259.
[15] 陈晓瑜, 陈峰, 张晨虹. 一株节食小鼠肠道优势鼠乳杆菌加速DSS致慢性结肠炎恢复[J]. 基因组学与应用生物学, 2021, 40(2): 855-866.

三种多酚类化合物对模拟间断性高原低氧暴露小鼠肠道菌群的影响^*

Effects of three Polyphenolic compounds on the intestinal flora of mice exposed simulated intermittent plateau hypoxia

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 12

编辑推荐

Metrics

本文评价

[1]	任彩佩, 张一楠, 吴亚俐, 都新新, 崔香丽. 白藜芦醇抑制肠癌细胞焦亡的作用^*[J]. 中国应用生理学杂志, 2022, 38(4): 326-331.
[2]	李林芳, 吕鑫钰, 邱玉玲, 孔德新. 基于网络药理学分析芪贞元丹治疗动脉粥样硬化的作用机制^*[J]. 中国应用生理学杂志, 2022, 38(3): 273-278.
[3]	胡佳楠, 姜辉, 李美琦, 朱俐, 骆倩倩. 模拟高原低氧环境对小鼠脾脏铁代谢的影响^*[J]. 中国应用生理学杂志, 2021, 37(6): 628-631.
[4]	许逸岚, 徐炀, 李鸿飞, 沈铄恒, 阮文丽, 郭晓欢, 赵镘娜, 韩瑜颖, 张衡. 原花青素提高鱼藤酮诱导的帕金森模型细胞活力的研究^*[J]. 中国应用生理学杂志, 2020, 36(5): 424-427.
[5]	谢亚磊, 梅颂, 熊艳蕾, 刘诗颖, 徐成丽. 模拟海拔5 500 m低压低氧环境对大鼠HPT轴和肠道菌群的影响^*[J]. 中国应用生理学杂志, 2020, 36(5): 432-437.
[6]	孙晓娟, 冯武龙, 侯娜, 李娜, 姜蔚丽. 有氧运动和白藜芦醇对2型糖尿病大鼠肾脏JAK2及TGF-β1的影响[J]. 中国应用生理学杂志, 2020, 36(3): 202-206.
[7]	王素星, 魏金刚, 邵伟华, 吕彩霞, 苑晓烨. 白藜芦醇对成年期追赶生长大鼠体成分的影响及机制[J]. 中国应用生理学杂志, 2020, 36(1): 85-89.
[8]	刘鑫, 吴亚俐, 刘凯丽, 崔香丽, 都新新, 张文琴. 白藜芦醇对小鼠溃疡性结肠炎的影响^*[J]. 中国应用生理学杂志, 2019, 35(5): 447-453.
[9]	林琨, 陈佳濠, 方泽浩, 叶铖龙, 韩超杰, 严明, 方剑, 张云. 原花青素对磷酸三钙磨损颗粒所致小鼠颅骨溶解的干预作用及其机制^*[J]. 中国应用生理学杂志, 2019, 35(3): 250-255.
[10]	崔嘉琴, 柏友萍, 苏敏, 宋威巍, 林潺, 吴琳, 张皓月. 不同强度运动结合白藜芦醇对老年肥胖大鼠RBP4的影响[J]. 中国应用生理学杂志, 2017, 33(5): 461-465.
[11]	宋娟, 王佳, 李宝红, 王东, 孟董超, 卢彦珍. PI3K/Akt信号通路在白藜芦醇抗大鼠缺血/再灌注性心律失常中的作用及机制[J]. 中国应用生理学杂志, 2017, 33(3): 239-243.
[12]	卢彦珍, 王佳, 宋娟, 李宝红, 郭雅玲, 王东, 张翠英. NOTCH1信号通路介导白藜芦醇抗肝缺血/再灌注所致大鼠心脏损伤保护作用的机制研究[J]. 中国应用生理学杂志, 2016, 32(4): 351-355.