耐力运动增强小鼠骨骼肌葡萄糖摄取能力的作用及白细胞介素15的调控机制*

doi:10.12047/j.cjap.6360.2022.126

中国应用生理学杂志 ›› 2022, Vol. 38 ›› Issue (6): 696-699.doi: 10.12047/j.cjap.6360.2022.126

耐力运动增强小鼠骨骼肌葡萄糖摄取能力的作用及白细胞介素15的调控机制^*

王哲^1,2, 王谦¹, 吴华朵¹, 胡永强¹, 彭子富¹, 郭项英¹, 姜宁^1△

1.天津体育学院运动健康学院, 天津 301617;
2.武警后勤学院基础部共同科目教研室, 天津 300309

收稿日期:2022-07-11 修回日期:2022-11-22 出版日期:2022-11-28 发布日期:2023-06-12
通讯作者: ^△Tel: 18822623099; E-mail: ningjiangtj@163.com
基金资助:
^*国家自然科学基金面上项目(31370021)

Effects of endurance exercise on glucose uptake of skeletal muscle in mice and regulation mechanism of interleukin 15

WANG Zhe, WANG Qian, WU Hua-duo, HU Yong-qiang, PENG Zi-fu, GUO Xiang-ying, JIANG Ning

Received:2022-07-11 Revised:2022-11-22 Online:2022-11-28 Published:2023-06-12

摘要/Abstract

摘要： 目的: 探讨耐力运动对小鼠骨骼肌葡萄糖摄取能力的影响及白细胞介素(IL-15)的作用机制。方法: 将32只C57BL/6J小鼠随机分为安静对照组(Control, C)、耐力训练组(Exercise, E)、PBS注射组(PBS injected, PI)、IL-15注射组(IL-15 injected, II)。每组8只,C组小鼠正常饲养8周,E组小鼠进行8周中等强度耐力训练,II组小鼠一次性腹腔注射300 μl IL-15复合剂溶液(1.5 μg IL-15和7 μg IL-15Rα),PI组一次性腹腔注射与II组等量的PBS。其中C组和E组在饲养第7周时,PI组和II组在一次性注射后,各组小鼠禁食12 h,随后进行葡萄糖耐量试验(IPGTT)检测并记录血糖水平。采用Western blot检测小鼠骨骼肌GLUT4、IL-15、AKT、pAKT、AMPK、pAMPK、AS160、pAS160的蛋白表达水平。结果: 与C组相比,E组小鼠骨骼肌IL-15、GLUT4、pAMPK、pAKT、pAS160蛋白表达明显升高(P＜0.05)。与PI组相比,II组小鼠骨骼肌IL-15、GLUT4、pAMPK、pAS160蛋白表达明显升高(P＜0.05)。结论: 8周耐力运动和腹腔注射IL-15复合剂均可提升小鼠骨骼肌葡萄糖的摄取能力,但单独增加IL-15可能是通过激活AMPK/AS160/GLUT4通路提升对于葡萄糖的转运能力,这一过程不依赖AKT/AS160/GLUT4通路的激活。

关键词: 运动, IL-15, 小鼠, 骨骼肌, AKT/AS160/GLUT4通路, AMPK/AS160/GLUT4通路

Key words: exercise, IL-15, mice, skeletal muscle, AKT/AS160/GLUT4 pathway, AMPK/AS160/GLUT4 pathway

中图分类号:

G804.2

王哲, 王谦, 吴华朵, 胡永强, 彭子富, 郭项英, 姜宁. 耐力运动增强小鼠骨骼肌葡萄糖摄取能力的作用及白细胞介素15的调控机制^*[J]. 中国应用生理学杂志, 2022, 38(6): 696-699.

WANG Zhe, WANG Qian, WU Hua-duo, HU Yong-qiang, PENG Zi-fu, GUO Xiang-ying, JIANG Ning. Effects of endurance exercise on glucose uptake of skeletal muscle in mice and regulation mechanism of interleukin 15[J]. CJAP, 2022, 38(6): 696-699.

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耐力运动增强小鼠骨骼肌葡萄糖摄取能力的作用及白细胞介素15的调控机制^*

Effects of endurance exercise on glucose uptake of skeletal muscle in mice and regulation mechanism of interleukin 15

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