小鼠原发性肝癌模型的建立*

doi:10.12047/j.cjap.6367.2022.149

中国应用生理学杂志 ›› 2022, Vol. 38 ›› Issue (6): 820-823.doi: 10.12047/j.cjap.6367.2022.149

• 技术方法 • 上一篇

小鼠原发性肝癌模型的建立^*

王靳琎¹, 李雪莹², 易进科³, 赵蓓玲⁴, 黄慧敏¹, 魏英^1△

1.湖北医药学院附属国药东风总医院实验中心, 十堰 442008;
2.湖北医药学院附属国药东风总医院病理科, 十堰 442008;
3.湖北医药学院附属国药东风总医院康复科, 十堰 442008;
4.湖北医药学院第二临床学院, 十堰 442008

收稿日期:2022-10-14 修回日期:2022-11-25 出版日期:2022-11-28 发布日期:2023-06-12
通讯作者: ^△Tel: 0719-8272534; E-mail: 57729968@qq.com
基金资助:
^*湖北省自然科学基金(2019CFB429); 十堰市科技基金(21Y74); 湖北省卫健委项目(WJ2021F054); 湖北医药学院2021年大学生创新创业训练计划项目(X202110929020); 国药东风总医院院内立项(2021Q6)

Establishment of primary liver cancer model in mice

WANG Jin-jin¹, LI Xue-ying², YI Jin-ke³, ZHAO Bei-ling⁴, HUANG Hui-min¹, WEI Ying^1△

1. Experiment Center of Medicine, Sinopharm Dongfeng General Hospital, Hubei University of Medicine, Shiyan 442008;
2. Department of Pathology, Sinopharm Dongfeng General Hospital, Hubei University of Medicine, Shiyan 442008;
3. Department of Rehabilitation, Sinopharm Dongfeng General Hospital, Hubei University of Medicine, Shiyan 442008;
4.The Second Clinical College, Hubei University of Medicine, Shiyan 442008, China

Received:2022-10-14 Revised:2022-11-25 Online:2022-11-28 Published:2023-06-12

摘要/Abstract

摘要： 目的: 采用三种造模方法建立小鼠原发性肝癌模型,并进行比较,寻找更优化的造模方法。方法: 15日龄C3H/HeN雄性小鼠40只,随机分为Ⅰ-Ⅳ组,每组10只。Ⅰ组:不予任何处理;Ⅱ组:腹腔注射25 mg/kg的二乙基亚硝胺(DEN)一次;Ⅲ组:腹腔注射100 mg/kg的DEN一次;Ⅳ组:腹腔注射25 mg/kg的DEN一次,42日龄时再次腹腔注射100 mg/kg的DEN一次。统计各组小鼠的死亡率。造模第18周,麻醉后眼球取血,断颈处死后打开腹腔取肝脏,观察各组肝脏外观、癌结节数目及肝脏肿瘤发生率,HE染色观察肝脏组织病理学变化,并检测血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)的浓度。结果: 造模第18周,与Ⅰ组相比,Ⅱ-Ⅳ组小鼠血清中的ALT 、AST浓度均显著升高(P＜0.05);Ⅲ组和Ⅳ组存活小鼠癌结节数目,肿瘤发生率显著增加(P＜0.05)。造模第18周时,Ⅰ组和Ⅱ组无小鼠死亡,肝癌发生率为0%;Ⅲ组和Ⅳ组存活小鼠肝癌发生率均为100%,但Ⅲ组小鼠死亡率高达50%,Ⅳ组仅为20%。结论: C3H/HeN雄性小鼠15日龄时腹腔注射25 mg/kg的DEN一次,第42日龄时再次腹腔注射100 mg/kg的DEN一次,可成功建立小鼠肝癌模型,周期短且死亡率较低,是建立原发性肝癌模型较为理想的方法。

关键词: 原发性肝癌, C3H/HeN小鼠, 二乙基亚硝胺, 谷丙转氨酶, 谷草转氨酶

Abstract: Objective: Three modeling methods were used to establish a mouse primary liver cancer model, and compared them to find a more optimal modeling method. Methods: Forty 15-day-old C3H/HeN male mice were randomly divided into groups I-IV, 10 mice in each group. Group Ⅰ were not treated; Group Ⅱ were intraperitoneally injected with 25 mg/kg diethylnitrosamine (DEN) once; Group Ⅲ were intraperitoneally injected with 100 mg/kg DEN once; Group Ⅳ were intraperitoneally injected with 25 mg/kg DEN once and followed by another intraperitoneal injection of 100 mg/kg DEN at 42 days of age. The mortality of mice in each group was analyzed. At the 18th week of modeling, blood was collected from eyeballs after anesthesia, and liver was taken from abdominal cavity after neck was broken. The appearance of liver, the number of cancer nodules and the incidence of liver tumor were observed. The histopathological changes of liver were observed by HE staining. The serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were detected. Results: At the 18th week of modeling, compared with the group I, serum levels of ALT and AST in groups II-IV were increased significantly (P＜0.05); The number of cancer nodules and the incidence of tumors in the surviving mice of groups III and IV were also increased significantly (P＜0.05). At the 18th week of modeling, no mice died in both groups I and II, and the incidence of liver cancer was 0%; The incidence of liver cancer in surviving mice in both groups III and IV was 100%, but the mortality rate of mice in group III was as high as 50%, and that in group IV was only 20%. Conclusion: C3H/HeN male mice can successfully establish a mouse liver cancer model by intraperitoneal injection of 25 mg/kg of DEN once at the age of 15 days and another intraperitoneal injection of 100 mg/kg of DEN once at the age of 42 days with short cycle and low mortality, which is an ideal method to establish a primary liver cancer model.

Key words: primary liver cancer, C3H/HeN mice, diethylnitrosamine, alanine transaminase, aspartate aminotransferase

中图分类号:

R332

王靳琎, 李雪莹, 易进科, 赵蓓玲, 黄慧敏, 魏英. 小鼠原发性肝癌模型的建立^*[J]. 中国应用生理学杂志, 2022, 38(6): 820-823.

WANG Jin-jin, LI Xue-ying, YI Jin-ke, ZHAO Bei-ling, HUANG Hui-min, WEI Ying. Establishment of primary liver cancer model in mice[J]. CJAP, 2022, 38(6): 820-823.

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小鼠原发性肝癌模型的建立^*

Establishment of primary liver cancer model in mice

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