不同强度运动训练对心肌凋亡途径微小RNA及其靶蛋白的影响

doi:10.12047/j.cjap.5502.2018.023

中国应用生理学杂志 ›› 2018, Vol. 34 ›› Issue (1): 93-96.doi: 10.12047/j.cjap.5502.2018.023

• 研究论文 • 上一篇

不同强度运动训练对心肌凋亡途径微小RNA及其靶蛋白的影响

赵永才¹, 付近梅², 高炳宏¹

1. 上海体育学院, 上海 200438;
2. 江西省体育科学研究所, 南昌 330006

收稿日期:2016-10-09 修回日期:2017-07-03 出版日期:2018-01-28 发布日期:2018-06-19
通讯作者: 高炳宏,Tel:021-51253167;E-mail:gaobinghong@126.com E-mail:gaobinghong@126.com
基金资助:
上海市科学技术委员会重点攻关项目（15dz1208001）；上海市人类运动能力开发与保障重点实验室（17DZ2273100）

Effects of different intensity exercise training on apoptosis-related microRNAs and the targeted proteins in cardiomyocytes

ZHAO Yong-cai¹, FU Jin-mei², GAO Bing-hong¹

1. Shanghai University of Sport, Shanghai 200438;
2. Institute for Sports Science of Jiangxi Province, Nanchang 330006, China

Received:2016-10-09 Revised:2017-07-03 Online:2018-01-28 Published:2018-06-19
Supported by:
上海市科学技术委员会重点攻关项目（15dz1208001）；上海市人类运动能力开发与保障重点实验室（17DZ2273100）

摘要/Abstract

摘要： 目的：考察不同负荷运动训练对小鼠心肌凋亡相关miR-1，miR-21和靶蛋白的影响，探讨运动干预心肌凋亡的可能机制。方法：选取21只C57BL/6小鼠，随机分为3组（n=7）：安静组（SE组）、训练1组（ET1组）、训练2组（ET2）。SE组不进行训练，ET1组完成8周递增负荷游泳训练，5天/周，1次/天，第1周30 min/count，每周增加10 min，第7、8周时间维持在90 min；ET2组在ET1组方案基础上增加负荷，前5周与ET1相同，后3周每天训练2次。TUNEL检测考察心肌凋亡水平，Western blot和RT-PCR分别测定蛋白和miRs的变化。结果：ET1组游泳训练对小鼠心肌凋亡影响不明显，miR-1表达无显著变化，但其靶蛋白Bcl-2表达显著增高（P<0.01），miR-21及其靶蛋白PDCD4表达均无显著变化。ET2组游泳训练显著降低心肌凋亡水平及miR-1表达（P<0.01）、提高Bcl-2表达（P<0.05）；同时显著提高miR-21表达（P<0.05），但对PDCD4表达无明显影响。结论：ET1组训练对心肌凋亡干预不明显，ET2组运动训练可降低心肌凋亡水平，miR-1及靶蛋白Bcl-2变化可能是机制之一，PDCD4对运动训练不敏感，miR-21可能与其它靶蛋白参与运动干预心肌凋亡的分子机制。

关键词: 运动, 心肌, 微小RNA, 凋亡, 小鼠

Abstract: Objective: To detect the levels of miR-1, miR-21 and their targeted proteins in hearts of mice after different exercise training, and discuss potential molecular mechanism.Methods: Male C57BL/6 mice were randomly divided to 3 groups:sedentary (SE), exercise training 1(ET1) and exercise training 2 (ET2). SE did not do any exercise; ET1 undertook swimming training for 8 weeks, once a day, 5 days/week. Swimming 30 min in the 1^st week, and the duration was increased 10 min per week to 90 min and maintained in the 7^th and 8^th week. ET2 performed the same work as ET1 and switched to twice a day by the end of the 5th week. TUNEL assay was applied to test myocardial apoptosis. Western blot and RT-PCR were used to detect proteins and miRs levels respectively.Results: Compared with SE, in ET1, myocardial apoptosis and miR-1 level did not change, but its targeted protein Bcl-2 increased significantly(P<0.01). miR-21 and its targeted protein PDCD4 did not change significantly. In ET2, myocardial apoptosis and miR-1 level were decreased significantly(P<0.05). Bcl-2 was increased significantly(P<0.01). miR-21 also increased significantly (P<0.05), but PDCD4 did not decrease significantly.Conclusion: Exercise training in ET2 other than ET1 could down-regulate myocardial apoptosis. Alterations of miR-1 and Bcl-2 may be responsible for this cardioprotection. PDCD4 is not sensitive to exercise training, it is likely that miR-21 and other targeted proteins participate in exercise-regulative apoptosis.

Key words: exercise, cardiomyocyte, microRNAs, apoptosis, mice

赵永才, 付近梅, 高炳宏. 不同强度运动训练对心肌凋亡途径微小RNA及其靶蛋白的影响[J]. 中国应用生理学杂志, 2018, 34(1): 93-96.

ZHAO Yong-cai, FU Jin-mei, GAO Bing-hong. Effects of different intensity exercise training on apoptosis-related microRNAs and the targeted proteins in cardiomyocytes[J]. CJAP, 2018, 34(1): 93-96.

参考文献

[1] Porrello ER, Johnson BA, Aurora AB, et al. MiR-15 family regulates postnatal mitotic arrest of cardiomyocytes[J]. Circ Res, 2011, 109(6):670-679.
[2] Wasiak S, Zunino R, McBride HM. Bax/Bak promote sumoylation of DRP1 and its stable association with mitochondria during apoptotic cell death[J]. J Cell Biol, 2007, 177(3):439-450.
[3] Tang Y, Zheng J, Sun Y, et al. MicroRNA-1 regulates cardiomyocyte apoptosis by targeting Bcl-2[J]. Int Heart J, 2009, 50(3):377-387.
[4] Cheng Y, Zhu P, Yang J, et al. Ischaemic preconditioning-regulated miR-21 protects heart against ischaemia/reperfusion injury via anti-apoptosis through its target PDCD4[J]. Cardiovasc Res, 2010, 87(3):431-439.
[5] Ignarro LJ, Balestrieri ML, Napoli C. Nutrition, physical activity, and cardiovascular disease:an update[J]. Cardiovasc Res, 2007, 73(2):326-340.
[6] Huang CY, Yang AL, Lin YM, et al. Anti-apoptotic and pro-survival effects of exercise training on hypertensive hearts[J]. J Appl Physiol, 2012, 112(5):883-891.
[7] Fannin J, Rice KM, Thulluri S, et al. The effects of aging on indices of oxidative stress and apoptosis in the female Fischer 344/Nnia X Brown Norway/BiNia rat heart[J]. Open Cardiovasc Med J, 2013, 29(7):113-121.
[8] 金丽, 丁宁, 杨月琴. 有氧运动对肥胖和肥胖抵抗大鼠心肌细胞凋亡及其它影响的研究[J]. 华中师范大学学报(自然科学版), 2011, 45(3):456-461.
[9] Siu PM, Bryner RW, Martyn JK, et al. Apoptotic adaptations from exercise training in skeletal and cardiac muscles[J]. FASEB J, 2004, 18(10):1150-1152.
[10] Kwak HB, Song W, Lawler JM. Exercise training attenuates age-induced elevation in Bax/Bcl-2 ratio, apoptosis, and remodeling in the rat heart[J]. FASEB J, 2006, 20(6):791-793.
[11] Chien KR. Molecular medicine:microRNAs and the tell-tale heart[J]. Nature, 2007, 447(7143):389-390.
[12] Dong S, Cheng Y, Yang J, et al. MicroRNA expression signature and the role of microRNA-21 in the early phase of acute myocardial infarction[J]. J Biol Chem, 2009, 284(43):29514-29525.
[13] Duan X, Ji B, Wang X, et al. Expression of microRNA-1 and microRNA-21 in different protocols of ischemic conditioning in an isolated rat heart model l. Cardiol, 2012, 122(1):36-43.
[14] Buscaglia LE, Li Y. Apoptosis and the target genes of microRNA-21[J]. Chin J Cancer, 2011, 30(6):371-380.

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不同强度运动训练对心肌凋亡途径微小RNA及其靶蛋白的影响

Effects of different intensity exercise training on apoptosis-related microRNAs and the targeted proteins in cardiomyocytes

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