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中国应用生理学杂志 ›› 2018, Vol. 34 ›› Issue (5): 432-435.doi: 10.12047/j.cjap.5655.2018.098

• 研究论文 • 上一篇    下一篇

银杏叶提取物对对乙酰氨基酚诱导的小鼠急性肝损伤的保护作用

张全书1, 王翔鹏1, 谢燕妮1, 武璐璐1,2, 刘红1   

  1. 1. 湖北民族学院医学院, 恩施 445000;
    2. 风湿疾病发生与干预湖北省重点实验室, 湖北 恩施 445000
  • 收稿日期:2017-11-20 修回日期:2018-09-25 出版日期:2018-09-28 发布日期:2019-02-21
  • 通讯作者: 刘红,Tel:15997726926;E-mail:2548449651@qq.com E-mail:2548449651@qq.com
  • 基金资助:
    国家自然科学基金资助项目(81260458);湖北民族学院大学生创新创业训练计划资助项目(2016CX143)

Protective effect of Ginkgo biloba extract on paracetamol-induced acute hepatic injury in mice

ZHANG Quan-shu1, WANG Xiang-peng1, XIE Yan-ni1, WU Lu-lu1,2, LIU Hong1   

  1. 1. College of Medicine, Hubei University for Nationalities, Enshi 445000;
    2. Hubei Provincial Key Laboratory of Occurrence and Intervention of Rheumatic Diseases, Enshi 445000, China
  • Received:2017-11-20 Revised:2018-09-25 Online:2018-09-28 Published:2019-02-21
  • Supported by:
    国家自然科学基金资助项目(81260458);湖北民族学院大学生创新创业训练计划资助项目(2016CX143)

摘要: 目的:探究银杏叶提取物(GBE)对对乙酰氨基酚(APAP)诱导的小鼠急性肝损伤的保护作用及其机制。方法:30只小鼠随机分为对照组、模型组、GBE低、中、高剂量组(50,100,and 200 mg·kg-1),每组6只。除对照组外,剩余小鼠腹腔注射APAP (300 mg/kg)一次,随后GBE低、中、高剂量组按照相应剂量灌胃给药,治疗2 d后取材。观察各组肝脏大体情况和肝组织的病理组织学变化;取血测定各组小鼠血清中ALT、AST的活性和TNF-α、IL-6的水平;取肝检测各组肝组织中SOD、MPO的活性和GSH、MDA的含量;通过Western blot检测各组肝组织中Nrf2、HO-1蛋白的表达量。结果:与对照组相比,模型组肝脏明显肿大,病理表现差,血清中ALT、AST、TNF-α、IL-6的水平显著升高(P<0.01),肝组织中GSH的含量和SOD的活性显著降低(P<0.01),MDA的含量和MPO的活性显著升高(P<0.01),Nrf2、HO-1蛋白表达明显下调(P<0.01)。与模型组相比,GBE组肝脏肿大减轻,病理表现有所改善,血清中ALT、AST、TNF-α、IL-6的水平显著降低(P<0.01),肝组织中GSH的含量和SOD的活性显著提高(P<0.01),MDA的含量和MPO的活性显著降低(P<0.01),Nrf2、HO-1蛋白表达上调(P<0.05),其中高剂量GBE组治疗效果最明显。结论:GBE可对APAP诱导的小鼠急性肝损伤具有保护作用,其作用机制可能是通过Nrf2/HO-1抗氧化途径发挥作用。

关键词: 银杏叶提取物, 对乙酰氨基酚, 氧化应激, 小鼠, 肝损伤

Abstract: Objective: To investigate the protective effects of Ginkgo biloba extract(GBE) on paracetamol(APAP)-induced acute hepatic injury in mice and its mechanism. Methods: Thirty mice were randomly divided into control group, model group, GBE low, medium and high-dose(50,100,and 200 mg·kg-1)groups,with 6 mice in each group. All mice except control group were administered with APAP(300 mg/kg)for one time by intraperitoneal injection. The mice in GBE low, medium and high-dose groups were intragastric administered with GBE for 2 d consecutively, then samples were harvested for analysis. The appearance and pathology of liver were observed. The levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in serum and the levels of superoxide dismutase (SOD), myeloperoxidase(MPO), glutathione (GSH) and malondialdehyde (MDA) in hepatic tissue were measured. Western blot was used to detect the protein expressions of Nrf2 and HO-1. Results: Compared with control group, in model group, the appearance and pathology of liver were bad, the levels of ALT,AST,TNF-α and IL-6 in serum were increased significantly(P<0.01),the levels of GSH and SOD were decreased while the levels of MDA and MPO were increased in hepatic tissue(P<0.01), the expressions of Nrf2 and HO-1 were increased in hepatic tissue(P<0.05). Compared with model group, in GBE groups, the appearance and pathology of liver were improved, the levels of ALT,AST,TNF-α and IL-6 in serum were decreased significantly(P<0.01), the levels of GSH and SOD were increased while the levels of MDA and MPO were decreased in hepatic tissue(P<0.01), the expression of Nrf2 and HO-1 were increased in hepatic tissue(P<0.05). The high-dose of GBE possessed the most obvious treatment effect among them. Conclusion: GBE may play a protective role in APAP-induced acute hepatic injury through Nrf2/HO-1 pathway.

Key words: Ginkgo biloba extract, paracetamol, oxidative stress, mice, acute hepatic injury

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