黑苦荞茎叶对2型糖尿病小鼠的治疗作用及其对其胰腺、脾脏的影响*

doi:10.12047/j.cjap.5670.2019.031

摘要/Abstract

摘要： 目的: 观察黑苦荞茎叶(BBL)对2型糖尿病小鼠治疗作用及其对胰腺、脾脏的影响。方法: 将40只SPF级雄性C57/B16小鼠随机分为正常对照组(n＝10)和实验组(n=30),实验组给予高糖高脂饲料喂养1个月后,每只小鼠在禁食12 h 后腹腔注射链脲佐菌素35 mg/kg,注射后72 h 取尾血测血糖≥13.8 mmol/L 为糖尿病小鼠。成功建立2型糖尿病(T2DM)模型的30只小鼠随机分为3组(n=10):模型对照组(DM组)、低剂量黑苦荞茎叶治疗组(DM+L)组、高剂量黑苦荞茎叶治疗(DM+H)组。对照组和模型组小鼠每日给予生理盐水灌胃,两个黑苦荞茎叶组分别给予0.21 g/kg·d^-1和0.42 g/kg·d^-1的黑苦荞茎叶灌胃,14 d后杀死小鼠,取血和胰腺、脾脏,检测血清中葡萄糖(FBG)、甘油三酯(TG)、总胆固醇(TCH)、胰岛素和脾脏组织中TNF-α蛋白的含量,观察胰腺组织形态学,称量小鼠体重和脾脏重量、计算脾脏系数,检测胰腺组织中IRS-1 mRNA的表达和胰腺组织内胰岛素受体底物IRS-1蛋白表达。结果: 与对照组相比,模型组小鼠血清FBG、TC、TCH水平明显升高,使血胰岛素水平明显下降,脾脏组织TNF-α蛋白表达水平显著升高,胰腺组织中IRS-1 mRNA表达和IRS-1的蛋白表达水平显著降低(P＜0.05);与模型组相比,治疗组小鼠的血清FBG、TC、TCH水平明显降低, 血胰岛素水平显著升高,小鼠脾脏系数、脾脏组织TNF-α蛋白表达水平、胰腺组织IRS-1 mRNA表达和IRS-1的蛋白表达水平显著升高(P＜0.05)。高剂量黑苦荞茎叶(0.42 g/kg·d^-1)治疗作用更加明显。结论: 黑苦荞茎叶对2型糖尿病小鼠具有显著的降血糖、降血脂的治疗效果,对糖尿病小鼠胰腺、脾脏具有一定的保护作用。

关键词: 黑苦荞茎叶, 2型糖尿病, 小鼠, 胰腺, 脾脏

Abstract: Objective: To investigate the therapeutic effects of the black buckwheat leaf (BBL) in type 2 diabetes mellitus mice and its effects on pancreas and spleen. Methods: Forty male C57 / B16 mice (SPF) were randomly divided into normal control (NC) group (n=10) and the experimental group (n=30), the experimental group were fed with high sugar and high fat, combined with intraperitoneal injection of streptozotocin in small dose to establish the model of type 2 diabetes mellitus (T2DM). Those thirty model mice were randomly divided into 3 groups (n=10), diabetes mellitus group (DM), low dose of BBL (DM+L) treated group, high dose of BBL (DM+H) treated group. The mice in the NC group and the DM group were given normal saline per day, and the DM+L group and DM+H group were treated with black tartary buckwheat at the doses of 0.21g/kg·d^-1 and 0.42g/kg·d^-1 respectively. After 14 days. All mice were executed by cervical dislocation, then blood samples were collected, pancreas and spleen were removed for subsequent experiments. The serum levels of fasting blood glucose (FBG), triglyceride (TG), total cholesterol (TCH) and insulin were detected. TNF-α protein in spleen tissue was detected by ELISA kit. The morphology of pancreas tissue was observed by HE staining, and the spleen coefficient was calculated. The expression levels of insulin receptor substrate-1(IRS-1) mRNA and IRS-1 protein in pancreatic tissue were detected. Results: Compared with the control group, the serum levels of FBG, TC and TCH in the model group were increased significantly, while the serum level of insulin was decreased significantly (P＜0.05), the expression of TNF-α protein in spleen tissues was obviously raised, the expressions of IRS-1 mRNA and IRS-1 protein in pancreatic tissue in model group were decreased significantly (P＜0.05). Compared with the model group, the serum levels of FBG, TC and TCH were decreased significantly in the BBL treated groups. The serum insulin level, spleen coefficient, TNF-α protein expression level in spleen tissue, IRS-1 mRNA expression and IRS-1 protein expression levels in pancreatic tissue in BBL treated group were increased significantly (P＜ 0.05). High-dose black tartary buckwheat leaves (0.42g/kg·d^-1) exerted a more significant effect. Conclusion: Stem and leaf of black bitter buckwheat has significant therapeutic effects on reducing blood sugar and blood fat in type 2 diabetic mice, and has certain protective effects on pancreas and spleen of diabetic mice.

Key words: black buckwheat stem leaf, type 2 diabetes, mice, pancreas, spleen

项伟玲, 金丽琴, 高峰, 肖敏, 陈燕. 黑苦荞茎叶对2型糖尿病小鼠的治疗作用及其对其胰腺、脾脏的影响^*[J]. 中国应用生理学杂志, 2019, 35(2): 140-144.

XIANG Wei-ling, JIN Li-qin, GAO Feng, XIAO Min, CHEN Yan. Treatment of type 2 diabetes mellitus with the stem and leaf of blacktartary buckwheat and its effects on the pancreas and spleen[J]. CJAP, 2019, 35(2): 140-144.

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黑苦荞茎叶对2型糖尿病小鼠的治疗作用及其对其胰腺、脾脏的影响^*

Treatment of type 2 diabetes mellitus with the stem and leaf of blacktartary buckwheat and its effects on the pancreas and spleen

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