Home  About Journal Instructions for Authors Editorial Board Subscribe Advertisement Messages Board Contact Us 中文

CJAP ›› 2017, Vol. 33 ›› Issue (5): 431-435.doi: 10.12047/j.cjap.5553.2017.104

Previous Articles     Next Articles

Effects of yellow wine polyphenols on cardiomyocyte apoptosis in diabetic cardiomyopathy rats

PAN Sun-lei1,2, LIN Hui1,2, LUO Hang-qi2, GAO Fei-dan2, MENG Li-ping2, GUO Yan2, GUO Hang-yuan1,2, CHI Ju-fang1,2   

  1. 1. The First Clinical Medical College, Wenzhou Medical University, Wenzhou 325000;
    2. Department of Cardiology, Shaoxing People's Hospital, Shaoxing Hospital of Zhejiang University, Shaoxing 312000, China
  • Received:2017-01-16 Revised:2017-06-21 Online:2017-09-28 Published:2018-06-19
  • Supported by:
    浙江省科技厅公益项目(2016C33227);浙江省自然科学基金(LY14H020002);绍兴市公益项目(2015B70046);绍兴市公益项目(2014B70068)

Abstract: Objective: To investigate the effects of yellow wine polyphenols on the apoptosis of cardiomyocytes in diabetic cardiomyopathy rats.Methods: Thirty SD rats were randomly divided into control group (Control), diabetic cardiomyopathy group (DCM) and diabetic cardiomyopathy treated with yellow wine polyphenols group (DCM+YWP). A single intraperitoneal injection of 65 mg/kg streptozotocin (STZ) was utilized to establish a rat model of DCM. The rats in control group were treated with citrate buffer at the same dose of a single intraperitoneal injection. DCM+YWP group were treated with 18 mg/kg Yellow wine polyphenols by ig after modeling. After treated for 12 weeks, the general condition of rats were observed. The cardiac structure and function of the rats were observed by Doppler echocardiography. The ultrastructure of myocardium were observed using electron microscopy. The inflammation index of myocardial tissue was detected by enzyme-linked immunosorbent assay (ELISA). The oxidative stress in myocardial tissues was assessed by oxidative stress detection kits. The expressions of Bax, Bcl-2 and Caspase-3 (cleaved) in myocardial were detected by Western blot.Results: Compared with DCM group, the blood glucose levels and body weight of rats in the DCM+YWP group were not changed significantly. Echocardiography showed that left ventricular end-diastolic diameter, left ventricular end-systolic diameter were decreased (P<0.05), while fractional shortening and E/A ratio and Ea/Aa ratio were elevated (P<0.05). The levels of tumor factor-α(TNF-α), interleukin 1β(IL-1β) and interleukin 6(IL-6) in myocardium were decreased (P<0.05). The levels of oxidative stress malondiadehyde(MDA) were decreased and Superoxide dismutase(SOD), glutathione peroxidase(GSH-Px) were increased in myocardial tissue (P<0.05). The expression levels of Bax and Caspase-3 (cleaved) protein in myocardium were decreased (P<0.05), and the expression of Bcl-2 protein was increased (P<0.05).Conclusion: Yellow wine polyphenols can improve the diabetic cardiomyopathy rat cardiac function, attenuates inflammation and oxidative stress in diabetic rats, inhibit the apoptosis of cardiomyocytes in diabetic cardiomyopathy.

Key words: yellow wine polyphenols, diabetic cardiomyopathy, cardiomyocyte apoptosis, rat

Contact Email:editor@cjap.ac.cn
Copyright © 2015 CJAP, All Rights Reserved.
Powered by Beijing Magtech Co. Ltd