Effects and mechanisms of lotus leaf water extract on lipid metabolism of adult experimental obesity rats

doi:10.12047/j.cjap.5409.2017.114

CJAP ›› 2017, Vol. 33 ›› Issue (5): 476-480.doi: 10.12047/j.cjap.5409.2017.114

Effects and mechanisms of lotus leaf water extract on lipid metabolism of adult experimental obesity rats

LI Mao-mao¹, HUANG Xin-yuan¹, LIANG Qiang-kun², LI Yun-xia², YAN Yuan², TIAN Zhi-feng³, QIU Xiao-qing³, ZHANG Xiao-wen⁴, SONG Qing⁴, LI Hong-fang^2,5

1. The First Clinical Medical College of Lanzhou University, Lanzhou 730000;
2. Departmemt of Physiology, College of Basic Medicine, Lanzhou University, Lanzhou 730000;
3. Functional Laboratory of Medicine, Lanzhou University, Lanzhou 730000;
4. Guangzhou Institute of Sports Science, Guangzhou 510000;
5. Key Laboratory of Preclinical Study for New Traditional Chinese Medicine of Gansu Province, Lanzhou 730000, China

Received:2016-01-08 Revised:2017-07-12 Online:2017-09-28 Published:2018-06-19
Supported by:
教育部大学生创新创业项目（2014）；甘肃省中医药管理局科研项目（GZK-2015-23）；广州市体育科学研究合作项目（2013）

Abstract

Abstract: Objective: To investigate the lipid metabolic effect and mechanism of water extract of lotus leaves(Traditional Chinese Medicine).Methods: Isolated SD rat lipid tissues were suspended in organ baths containing Krebs solution, and the effect of lotus leaf water extract on free fatty acids (FFA) release was observed; The experimental obesity rat model was established by feeding them high glucose and fat diets, then the changes of body weight and blood lipid were measured in the model rats after intragastric administration with water extract of lotus leaves for four weeks, and the expressions of peroxisome proliferator-activated receptor gamma (PPAR-γ) and leptin were examined by RT-PCR and immunohistochemical.Results: The ex vivo experiment showed that water extract of lotus leaves effectively promoted the FFA release from isolated lipid tissues. In vivo experiment, similarly to Orlistat, water extract of lotus leaves(60 mg/kg)markedly decreased the body weight and blood lipid of experimental obesity rats(P<0.05), and obviously reduced the expressions of PPAR-γ and leptin(P<0.05).Conclusion: Water extract of lotus leaves greatly improves the expression of PPAR-γ and leptin, which can promote the lipid mobilization and dissolution, reduce the body weight and blood lipid of adult rats with experimental obesity, therefore is expected to be developed into lipid-lowering diet pills.

Key words: water extract of the lotus leaf, rats, obesity, PPAR-γ, leptin

LI Mao-mao, HUANG Xin-yuan, LIANG Qiang-kun, LI Yun-xia, YAN Yuan, TIAN Zhi-feng, QIU Xiao-qing, ZHANG Xiao-wen, SONG Qing, LI Hong-fang. Effects and mechanisms of lotus leaf water extract on lipid metabolism of adult experimental obesity rats[J]. CJAP, 2017, 33(5): 476-480.

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Effects and mechanisms of lotus leaf water extract on lipid metabolism of adult experimental obesity rats

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