Effects of resistance training on mitochondrial function in skeletal muscle of aging rats

doi:10.12047/j.cjap.5861.2020.037

Abstract

Abstract: Objective: To investigate the effects of resistance exercise on mitochondrial function in skeletal muscle of aging rats. Methods: Forty male Sprague-Dawley rats were randomly assigned to 4 groups, 2-month sedentary control group (C1; n=10), 2-month with resistance training group (R1; n=10), 6-month sedentary control group (C2; n=10), 6-month with resistance training group (R2; n =10 ). Rats in R1 and R2 groups were arranged for resistance training for 8 weeks. This program consisted of interval running on a treadmill, speed 15 m·min^-1, 35° incline, duration 15 s, interval 30 s, 4 times/group, 3 groups/cycle, 2 cycles per day, 6 days per week, a total of 8 weeks. The expressions of mitochondrial fusion protein 2(Mfn2) and dynamin-related protein 1(DRP1) in rat quadriceps were detected by Western blot, and the changes of mitochondrial membrane potential (ΔΨm), reactive oxygen species (ROS) and Ca²⁺ concentration were measured by flow cytometry. Results: ①Compared with C1 group, the expression of DRP1 protein in R1 group was increased (P＜0. 01), and the Mfn2 protein in R1 group had no significant difference, both DRP1 and Mfn2 protein in C2 group were decreased (P＜0. 01);compared with C2 group, the DRP1 and Mfn2 protein in R2 group were similarly increased (P＜0. 01, P＜0. 05);compared with R1 group, the DRP1 and Mfn2 protein in R2 group were both decreased (P＜0. 01). ② Compared with C1 group, the Ca²⁺ content of R1 group was decreased (P＜0. 01) and the Ca²⁺ content of C2 group was increased (P＜0. 01);Compared with C2 group, the content of Ca²⁺ in R2 group was decreased (P＜0. 01);compared with R1 group, the Ca²⁺ content in R2 group was increased (P＜0. 01). ③ Compared with C1 group, the ROS content in R1 group was increased, but there was no significant difference, while the ROS content in C2 group was increased (P＜0. 01);compared with C2 group, ROS content in R2 group was decreased (P＜0. 01); compared with R1 group, the ROS content in R2 group was increased (P＜0. 01). ④ Compared with group C1, the levels of ΔΨm in C2 group was decreased (P＜0. 01);Compared with C2 group, The ΔΨm of R2 group was increased(P＜0. 01); Compared with group R1, the ΔΨm of R2 group was decreased, but there was no statistical difference. Conclusion: During the aging process of rats, mitochondria of quadriceps femoral muscle showed Ca²⁺ accumulation, increased reactive oxygen species, decreased mitochondrial membrane potential, decreased fusion protein and other phenomena, and resistance training could effectively improve these changes.

Key words: rats, resistance training, quadriceps, mitochondria, fusion protein 2

CLC Number:

G804.7

SU Yan-hong, YUAN Qian-kun, XIAO Rong, CHEN Juan, LI Qiang, ZHANG Shi-chao. Effects of resistance training on mitochondrial function in skeletal muscle of aging rats[J]. CJAP, 2020, 36(2): 165-170.

References

[1] 康品方, 胡俊锋, 汤阳, 等. 低浓度乙醇对糖尿病大鼠心肌损伤后线粒体融合素2表达的影响[J]. 中国应用生理学杂志, 2016, 32(4): 347-350.
[2] 赵永才, 高炳宏. 线粒体动力学与细胞能量代谢的关系及运动干预研究进展[J]. 生理学报, 2019, 71(4): 625-636.
[3] 刘子泉. 线粒体融合与分裂的动态变化在增龄和耐力训练适应中的生理作用[D]. 天津体育学院, 2008.
[4] 王震, 林文弢, 翁锡全, 等. 抗阻训练对老龄大鼠腓肠肌Ca²⁺及线粒体膜电位的影响[J]. 广州体育学院学报, 2014, 34(2): 85-88.
[5] Fatouros IG, Kambas A, Katrabasas I, et al. Resistance training and detraining effects on flexibility performance in the elderly are intensity-dependent[J]. J Strength Cond Res, 2006, 20(3): 634-642.
[6] Haykowsky M, McGavoek J, Vonder Muhll I, et al. Effeet of exercise training on peak aerobic power, left ventricular morphology, and muscle strength in healthy oldwomen[J]. J Gerontol A BiolSci MedSci, 2005, 60(3): 307-310.
[7] 赵丽, 周巧霞, 王拴, 等. 线粒体分裂和融合相关蛋白质的研究进展[J]. 生理学报, 2018, 70(4): 424-432.
[8] 郑凯, 杨梅桂, 闫朝君, 等. 线粒体动力学与细胞凋亡[J]. 中国细胞生物学学报, 2019, 41(8): 1467-1476.
[9] Twig G, Elorza A, Molina AJ, et al. Fission and selective fusion govern mitochondrial segregation and elimination by autophagy[J]. EMBO J, 2008, 27(2): 433-446.
[10]Liu R, Jin P, Yu L, et al. Impaired mitochondrial dynamics and bioenergetics in diabetic skeletal muscle[J]. PLoS One, 2014, 9(3): e92810.
[11]Chen H, Vermulst M, Wang YE, et al. Mitochondrial fusion is required for mtDNA stability in skeletal muscle and tolerance of mtDNA mutations[J]. Cell, 2010, 141(2): 280-289.
[12]Chen Y, Csordás G, Jowdy C, et al. Mitofusin 2-containing mitochondrial-reticular microdomains direct rapid cardiomyocyte bioenergetic responses via interorganelle Ca²⁺ crosstalk[J]. Circ Res, 2012, 111(7): 863-875.
[13]王长青, 刘丽萍, 李雷, 等. 游泳训练后大鼠骨骼肌细胞自由基代谢、线粒体膜电位变化与细胞凋亡的关系[J]. 中国运动医学杂志, 2002, 21(3): 256-260.
[14]黄志辉, 刘浩, 李宪航. 有氧运动对衰老大鼠心肌、肝脏细胞凋亡及线粒体膜电位的影响[J]. 西安体育学院学报, 2007, 24(1): 78-80.
[15]Molina AJ, Bharadwaj MS, Van Horn C, et al. Skeletal muscle mitochondrial content, oxidative capacity, and Mfn2 expression are reduced in older patients with heart failure and preserved ejection fraction and are related to exercise intolerance[J]. JACC Heart Fail, 2016, 4(8): 636-645.
[16]Veeranki S, Givvimani S, Kundu S, et al. Moderate intensity exercise prevents diabetic cardiomyopathy associated contractile dysfunction through restoration of mitochondrial function and connexin 43 levels in db/db mice[J]. J Mol Cell Cardiol, 2016, 92: 163-173.
[17]林文弢, 王震, 翁锡全, 等. 抗阻训练对衰老大鼠线粒体介导腓肠肌细胞凋亡通路相关因子表达影响的研究[J]. 中国运动医学杂志, 2014, 33(10): 994-997.
[18]Porter C, Reidy PT, Bhattarai N, et al. Resistance exercise training alters mitochondrial function in human skeletal muscle[J]. Med Sci Sports Exerc, 2015, 47(9): 1922-1931.
[19]李洁, 谈文博, 王艳. 有氧运动对衰老大鼠骨骼肌线粒体能量代谢的影响[J]. 中国应用生理学杂志, 2018, 34(3): 234-237.
[20]Pietrangelo L, D'Incecco A, Ainbinder A, et al. Age-dependent uncoupling of mitochondria from Ca²⁺release units in skeletal muscle[J]. Oncotarget, 2015, 6(34): 35358-35371.