间歇运动对心梗大鼠梗死周边区单个心肌细胞钙瞬变和收缩功能的影响*

doi:10.12047/j.cjap.5721.2019.027

摘要/Abstract

摘要： 目的: 探讨间歇运动对心梗大鼠缺血心肌细胞钙瞬变和收缩功能改变的影响。方法: 3月龄SD雄性大鼠24只,适应性喂养1周后随机分为假手术组(S)、心梗组(MI)、心梗+运动组(ME),每组8只。MI组结扎左冠状动脉前降支制备心梗模型;S组只穿线不结扎;ME组术后一周开始间歇训练,运动方式为1周适应性运动(10 m/min×30 min/d),然后先以10 m/min×10 min,再以15 m/min×6 min和25 m/min×4 min依次交替运功,60 min/d,每周5 d连续8周。训练结束后次日,腹腔麻醉并分离心肌细胞。采用单细胞可视化动缘探测系统(IonOptix)测定[Ca²⁺]_i amplitude、[Ca²⁺]_i荧光比率(Ratio)、Departure velocity、TTP、TTP50％、TTB50％、Return velocity以及Ratio amplitude等钙瞬变指标和±dl/dtmax、SL、PTA、SL shortening％等心肌细胞收缩指标。结果: 与S组相比,MI组[Ca²⁺]_iamplitude、[Ca²⁺]_i Ratio amplitude,Departure velocity和Return velocity均显著下降(P＜0.01),TTB50％、TTP和TTP50％均显著增加(P＜0.01),心肌细胞肌节SL Shortening％、PTA、±dl/dtmax均显著减少(P＜0.01);与MI组相比,ME组Ratio amplitude、[Ca²⁺]_i amplitude、Return velocity和Departure velocity均显著提高(P＜0.01),TTB50％、TTP和TTP50％均显著缩短(P＜0.01,P＜0.05),心肌细胞肌节SL Shortening％、PTA、±dl/dtmax均显著提高(P＜ 0.01,P＜0.05)。结论: 间歇运动可同步改善MI大鼠梗死周边区心肌活细胞的钙瞬变异常和心肌细胞的收缩功能。

关键词: 钙瞬变, 心肌梗死, 收缩功能, 间歇运动, 大鼠

Abstract: Objective: To investigate the effects of interval training on calcium transient and contractile function in ischemic ventricular myocytes of rats with myocardial infarction and their synchronization. Methods: Twenty-four male sprague-dawley rats in three years old, were randomly divided into three groups (n=8): sham-operated group(S), sedentary MI group(MI) and MI with interval training group (ME). The MI model was established by ligation of the left anterior descending coronary artery. The rats in ME group started training 1 week after MI operation. The S model was established by threading only without ligation. ME model took one week adaptive training, 10 m/min and 30 min/d, then took subsequently 8-week aerobic interval training: 10 min×10 m/min, then reran the rats with 2 intensities 15 m/min×6 min and 25 m/min×4 min, 1 h/d, 5 d/week. After training 24 hours, the cardiomyocytes of all groups were isolated by using the Langendorff fusion system. The contractile function and calcium transient of single ventricular myocyte in myocardial infarction adult rats were detected by IonOptix. Calcium transients were measured as [Ca²⁺]_i amplitude, departure velocity, ratio, TTB50％, TTP and TTP50%, return velocity and ratio amplitude. PTA, SL,±dl/dtmax and SL shortening% were tested to evaluate contractility. Results: Compared with S, the levels of [Ca²⁺]_iamplitude, departure velocity, ratio amplitude and return velocity, SL shortening%, PTA and±dl/dtmax of MI were decreased(P＜0.01), the levels of TTP, TTP50% and TTB50% of MI were increased(P＜0.01); Compared with MI, the levels of departure velocity, ratio amplitude, return velocity and [Ca²⁺]_i amplitude of ME were increased(P＜0.01), the levels of TTB50%, TTP and TTP50% of ME were decreased(P＜0.01, P＜0.05). The levels of SL shortening%, PTA and±dl/dtmax of ME were increased(P＜0.01, P＜0.05). Conclusion: Interval training can improve calcium transient and contractile function of single ventricular myocyte in myocardial infarction adult rats.

Key words: calcium transient, myocardial infarction, contractile function, interval training, rat

薄文艳, 李达刚, 田振军. 间歇运动对心梗大鼠梗死周边区单个心肌细胞钙瞬变和收缩功能的影响^*[J]. 中国应用生理学杂志, 2019, 35(2): 121-125.

BO Wen-yan, LI Da-gang, TIAN Zhen-jun. Effects of interval training on calcium transient and contractile function of single ventricular myocyte in myocardial infarction adult rats[J]. CJAP, 2019, 35(2): 121-125.

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间歇运动对心梗大鼠梗死周边区单个心肌细胞钙瞬变和收缩功能的影响^*

Effects of interval training on calcium transient and contractile function of single ventricular myocyte in myocardial infarction adult rats

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