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中国应用生理学杂志 ›› 2016, Vol. 32 ›› Issue (4): 370-372.doi: 10.13459/j.cnki.cjap.2016.04.021

• 研究论文 • 上一篇    下一篇

槟榔碱对人乳腺癌MCF-7细胞增殖和凋亡的影响

奉水东1, 伍迪2, 杨丝丝2, 何剑琴2, 张恺芳2, 凌宏艳2   

  1. 1. 南华大学公共卫生学院社会医学与卫生事业管理学教研室, 湖南 衡阳 421001;
    2. 南华大学医学院生理学教研室, 湖南 衡阳 421001
  • 收稿日期:2015-06-08 修回日期:2016-02-29 出版日期:2016-07-28 发布日期:2018-06-20
  • 通讯作者: 凌宏艳,Tel:86-0734-8281389;E-mail:linghongyan0203@126.com E-mail:linghongyan0203@126.com
  • 基金资助:
    教育部留学归国基金(教外司留[2014]1685号);中国博士后科学基金(2012M511384);湖南省科技厅(2015JC3085)项目资助;南华大学留学归国基金项目(2013XQD49)

Effect of arecoline on proliferation and apoptosis of MCF-7 human breast cancer cells

FENG Shui-dong1, WU Di2, YANG Si-si2, HE Jian-qin2, ZHANG Kai-fang2, LING Hong-yan2   

  1. 1. Department of Social Medicine and Health Service Management, School of Public Health, Hengyang 421001, China;
    2. Department of Physiology, School of Medicine, University of South China, Hengyang 421001, China
  • Received:2015-06-08 Revised:2016-02-29 Online:2016-07-28 Published:2018-06-20
  • Supported by:
    教育部留学归国基金(教外司留[2014]1685号);中国博士后科学基金(2012M511384);湖南省科技厅(2015JC3085)项目资助;南华大学留学归国基金项目(2013XQD49)

摘要: 目的:观察槟榔碱对人乳腺癌细胞(MCF-7)增殖和凋亡的影响,并探讨其机制。方法:采用四甲基偶氮唑盐(MTT)法检测不同浓度(0、10、30、50、100、300、500μmol/L)槟榔碱对MCF-7细胞增殖的影响,Hoechst 33342染色和流式细胞术检测细胞凋亡,Western blot法检测Bax,Bcl-2和P53蛋白表达。结果:低浓度(0、10、30、50μmol/L)槟榔碱不影响细胞的增殖和凋亡;而高浓度(100、300、500 μmol/L)槟榔碱呈浓度依赖性抑制MCF-7细胞增殖、诱导MCF-7细胞凋亡、提高P53和Bax蛋白表达、降低Bcl-2蛋白表达。结论:高浓度槟榔碱抑制MCF-7细胞增殖、诱导凋亡,其机制可能与提高P53和Bax蛋白表达,降低Bcl-2蛋白表达有关。

关键词: 槟榔碱, MCF7细胞, 增殖, 凋亡

Abstract: Objective:To observe the effects of arecoline on proliferation and apoptosis of MCF-7 human breast cancer cells and to ex-plore its possible mechanism. Methods:Human breast cancer MCF-7 cells were treated with arecoline at the concentrations of 0,10,30,50, 100,300,500μmol/L, the cell proliferation were detected by MTT assay, cell apoptosis were analyzed by Hoechst 33342 staining and flow cy-tometry, the protein expression of Bax,Bcl-2 and P53 were detected by Western blot. Results:Low concentration(0,10,30, 50 μmol/L) arecoline had no effect on the proliferation and apoptosis of MCF-7. However, high concentration(100,300,500μmol/L) arecoline inhibited proliferation and induced apoptosis of MCF-7 cells in a concentration-dependent manner, arecoline also significantly increased P53 and Bax protein expression and decreased Bcl-2 protein expression. Conclusion:High concentration arecoline inhibited the proliferation and induced the apoptosis of MCF-7 cells, the mechanism was probably corrected with increasing P53 and Bax protein expression and decreasing Bcl-2 pro-tein expression.

Key words: arecoline, MCF-7 cells, proliferation, apoptosis

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