Effects of aminooxyacetic acid on the learning and memory ability and its possible mechanism in rats with chronic alcoholism

doi:10.12047/j.cjap.5662.2018.108

CJAP ›› 2018, Vol. 34 ›› Issue (6): 485-489.doi: 10.12047/j.cjap.5662.2018.108

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Effects of aminooxyacetic acid on the learning and memory ability and its possible mechanism in rats with chronic alcoholism

QIN Hao-zhi¹, JIANG Hong-bo^1,3, DAI Xuan¹, HUANG Ya-di¹, LUO Xiao-qiu¹, ZHANG Rui-ling^1,2, DU Ai-lin^1,2

1. Department of Physiology and Neurobiology and Sino-UK Joint Laboratory for Brain Function and Injury, Henan Provincial Key Laboratory of Brain Research, Xinxiang Medical University, Xinxiang 453003;
2. The Second Affiliated Hospital of Xinxiang Medical University, Key Laboratory of Biological Psychiatry in Henan Province, Xinxiang 453003;
3. The Third Affiliated Hospital of Xinxiang Medical University, Xinxiang 453003, China

Received:2017-11-29 Revised:2018-11-05 Online:2018-11-28 Published:2019-03-09
Supported by:
国家自然科学基金（81471351）；河南省教育厅课题（15A310006）；河南省卫生科技创新型人才工程专项经费（3052）；河南省高校科技创新团队（17IRTSTHN023）

Abstract

Abstract: Objective: To investigate the effects of aminooxyacetic acid (AOAA) on learning and memory ability and possible mechanisms in rats with chronic alcoholism.Methods: Sixty SD male rats were randomly divided into three groups on average.The model group rats and the remedy group rats were fed with the water containing (v/v) 6% alcohol for 28 days.After 14 days, the remedy group rats were treated with AOAA (5 mg/kg·d) by intraperitoneal injection once a day for 14 days and the other two group rats were treated with the equal amount of saline by intraperitoneal injection every day.Five days before the end of the experiment, the water maze test was carried out to test the learning and memory ability of rats for 5 days.Subsequently, the content of H₂S, the activity of ATP enzyme and the expression of 5-HT in hippocampus were measured.Results: Compared with the rats in the control group, the latency and the swimming distance of the 2nd to the 4th day, the content of H₂S in hippocampus of rats in the model group were all increased, the mitochondrial ATP enzyme activity in hippocampus and the positive expression of 5-HT in hippocampus CA1 and CA3 of rats in the model group were decreased (P<0.01).Compared with the rats in the model group, the latency and the swimming distance of the 2nd to the 4th day, the content of H₂S in hippocampus of the rats in the remedy group were decreased, the mitochondrial ATP enzyme activity in hippocampus and the positive expression of 5-HT in hippocampus CA1 and CA3 of rats in the model group were increased (P<0.01).Conclusion: AOAA could alleviate the symptoms of chronic alcoholism rats, which may be related to the effects of AOAA on the content of H₂S, the mitochondrial enzyme activity and the expression of 5-HT in hippocampus.

Key words: aminooxyacetic acid, chronic alcoholism, rats, 5-HT, mitochondria, learning and memory abilities

QIN Hao-zhi, JIANG Hong-bo, DAI Xuan, HUANG Ya-di, LUO Xiao-qiu, ZHANG Rui-ling, DU Ai-lin. Effects of aminooxyacetic acid on the learning and memory ability and its possible mechanism in rats with chronic alcoholism[J]. CJAP, 2018, 34(6): 485-489.

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Effects of aminooxyacetic acid on the learning and memory ability and its possible mechanism in rats with chronic alcoholism

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