Excitatory and aversive behaviors - electrophysiological observation of anterior cingulate cortex (ACC) in rats

doi:10.12047/j.cjap.5840.2019.105

Abstract

Abstract: Objective: Increasing activities in anterior cingulate cortex (ACC) can enhance the aversion reactions associated with noxious stimuli. It has been known that opioid receptors activation can trigger endogenous analgesic effect. This study tried to explore whether opioid receptors activation in the ACC region could reduce the aversion associated with noxious stimuli. Methods: The experimental rats were randomly divided into seven groups, Complete Freund's adjuvant (CFA) + normal saline (NS) group, normal saline (NS) + normal saline (NS) group, normal saline (NS) +DAMGO ((DAla², NMe-Phe⁴, Gly-ol⁵)enkephinlin, μ-opioid receptor agonist) group, complete Freund's adjuvant (CFA)+ 0.01/0.04/0.2/1 μg/μl DAMGO group(n=6). The experimental period was three days. The basal value was measured on the first day. The second day, 1 μl was administered through the ACC area, and then 0.08 ml of complete Freund's adjuvant (CFA) was injected into the left hind paw of the rat. CPA response, paw withdrawal reflex latency (PWL) and electrical activity in the ACC brain region of rats were observed on the third day. Results: ①PWL was significantly decreased in rats after CFA was injected into left hind paw compared with post-injection(P＜0.05).② In the pain side of the apparatus, it took rats less residence time than that in the non-pain side. ③ 0.04/0.2/1 μg/μl DAMGO was given before CFA-injection, C-CPA reactions could be revised significantly. ④ Given 0.04/0.2/1 μg/μl DAMGO in the ACC region could decrease the increasing discharge frequency induced by CFA in ACC neurons. Conclusion: The activation of the mu-opioid receptor in the ACC region alleviates the aversion induced by noxious stimulation.

Key words: anterior cingulate cortex (ACC), μ-opioid receptor, multi-channel electrophysiological technique, pain related aversion, rat

CLC Number:

R338.8

GUAN Xiao-ya, ZHANG Xiao-yi, LI Zhen-hua, QIN Xia, DU Zhen-ying, ZHAO Xin, LI Jian-guo, ZHANG Ce, ZHANG Yu. Excitatory and aversive behaviors - electrophysiological observation of anterior cingulate cortex (ACC) in rats[J]. CJAP, 2019, 35(6): 481-485.

References

[1] Williams AC, Craig KD. Updating the definition of pain[J]. Pain, 2016, 157: 2420-2423.
[2] Niederberger E, Resch E, Parnham MJ, et al. Drugging the pain epigenome[J]. Nat Rev Neurol, 2017, 13(7): 434-447.
[3] Dahlke AM, Sable JJ, Andrasik F. Behavioral therapy: emotion and pain, a common anatomical background[J]. Neurological Sciences, 2017, 38(1 Suppl): 157-161.
[4] Johansen JP, Fields HL, Manning BH. The affective component of pain in rodents: direct evidence for a contribution of the anterior cingulate cortex[J]. Proc Nat Acad Sci, 2001, 98(14): 8077-8082.
[5] Bodnar RJ. Endogenous opiates and behavior: 2015[J]. Peptides, 2017, 88: 126-188.
[6] 李振华, 杨洋, 侯苗苗, 等. 在体多通道电生理记录技术在大鼠痛情绪研究中的应用[J]. 中国应用生理学杂志, 2016, 32(1): 41-45.
[7] 付彤童, 徐静, 万燕杰, 等. 痛厌恶情绪对疼痛的影响及其可能机制[J]. 中国疼痛医学杂志, 2016, 22(6): 428-435.
[8] Kalivas PW, Abhold R. Enkephalin release into the ventral tegmental area in response to stress: modulation of mesocorticolimbic dopamine[J]. Brain Research, 1987, 414(2): 339-348.
[9] Zhang Y, Meng X, Li A, et al. Electroacupuncture alleviates affective pain in an inflammatory pain rat model[J]. Eur J Pain, 2012, 16(2): 170-181.
[10]Bannon MJ, Elliott PJ, Alpert JE, et al. Role of endogenous substance P in stress-induced activation of mesocortical dopamine neurones[J]. Nature, 1983, 306(5945): 791-792.
[11]Zhang Y, Li A, Xin J, et al. Involvement of spinal serotonin receptors in electroacupuncture anti-hyperalgesia in an inflammatory pain rat model[J]. Neurochem Res, 2011, 36(10): 1785-1792.
[12]Liu LY, Zhang RL, Chen L, et al. Chronic stress increases pain sensitivity via activation of the rACC-BLA pathway in rats[J]. Exp Neurol, 2019, 313: 109-123.
[13]唐雨龙, 张玉秋. 前扣带皮层NMDA受体-MAPK-CREB通路参与痛厌恶情绪的分子机制[J]. 生理学报, 2017, 69(5): 637-646
[14]Chen W, Ennes HS, McRoberts JA, et al. Mechanisms of μ-opioid receptor inhibition of NMDA receptor-induced substance P release in the rat spinal cord[J]. Neuropharmacology, 2018, 128: 255-268.
[15]朱贺, 马克涛, 李丽, 等. 神经病理痛大鼠DRG神经元GABA_A受体激活电流的变化[J]. 中国应用生理学杂志, 2011, 27(3): 376-379.
[16]Zhang Y, Ji F, Wang G, et al. BDNF activates mTOR to upregulate NR2B expression in the rostral anterior cingulate cortex required for inflammatory pain-related aversion in rats[J]. Neurochem Res, 2018, 43(3): 681-691.
[17]Gorka SM, Fitzgerald DA, de Wit H, et al. Opioid modulation of resting-state anterior cingulate cortex functional connectivity[J]. J Psychopharmacol, 2014, 28(12): 1115-1124.
[18]Navratilova E, Xie JY, Meske D, et al. Endogenous opioid activity in the anterior cingulate cortex is required for relief of pain[J]. J Neurosci, 2015, 35(18): 7264-7271.
[19]Vogt BA, Sikes RW. The medial pain system, cingulate cortex, and parallel processing of nociceptive information[J]. Prog Brain Res, 2000, 122: 223.
[20]Xie CW, Lewis DV. Involvement of cAMP-dependent protein kinase in μ-opioid modulation of NMDA-mediated synaptic currents[J]. J Neurophysiol, 1997, 78(2): 759-766.