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CJAP ›› 2016, Vol. 32 ›› Issue (4): 343-346.doi: 10.13459/j.cnki.cjap.2016.04.015

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A method of primary cell culture of rat pulmonary artery smooth muscle cells and effects of platelet-derived growth factor induced proliferation and migration

ZHU Ning, ZHAO Xu-yong, XIANG Yi-jia, ZENG Chun-lai   

  1. Cardiovascular Department, the Fifth Affiliated Hospital, Wenzhou Medical University, Lishui 323000, China
  • Received:2015-09-28 Revised:2016-05-16 Online:2016-07-28 Published:2018-06-20
  • Contact: 曾春来,Tel:0578-2681018;E-mail:zengchunlai@medmail.com.cn E-mail:zengchunlai@medmail.com.cn
  • Supported by:
    曾春来,Tel:0578-2681018;E-mail:zengchunlai@medmail.com.cn

Abstract: Objective:To establish an easy, not depending on advanced laboratory apparatus method to isolate and culture rat pulmonary artery smooth muscle cells (PASMCs), and to explore the effects of platelet-derived growth factor (PDGF) on cell proliferation and migration. Methods:The right ventricle was perfused with the mixture of iron, agarose, and the PASMCs and iron could adhere to agarose. The iron-con-taining tissue would move to side of the tube next to the magnet and could be digested by collagenase I. By the method, vessel-containing tissue could be attained. With 3-4 weeks' purification, the PASMCs could be obtained. The PASMCs morphology was observed by an inverted micro-scope, and identified by immunocytochemistry and immunofluorescence. The effects of PDGF on cell proliferation and migration was detected by MTT assay and scratch wound assay. Results:14 days、21 days and primary culture after isolation, the PASMCs was identified, and the re-sult showed that isolation and primary culture of the cells were PASMCs. Compared with the cells with no stimulation, the proliferation of PASMCs exposed to PDGF was increased significantly(P<0.05), and scratch wound assay demonstrated that PDGF induced the significant increase of migration of PASMCs. Conclusion:This method to isolate and culture rat PASMCs is simple, not depending on advanced laborato-ry. PDGF can promote the proliferation and migration of PASMCs.

Key words: pulmonary arterial smooth muscle cells, rat, isolation, primary cell culture

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