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中国应用生理学杂志 ›› 2016, Vol. 32 ›› Issue (2): 128-131.doi: 10.13459/j.cnki.cjap.2016.02.009

• 研究论文 • 上一篇    下一篇

二十二碳六烯酸对心房颤动大鼠心房肌生理改变及双孔钾通道TASK-1蛋白表达的影响

葛敏1, 单锋1,2, 唐碧3, 侯秀杰4   

  1. 1. 蚌埠医学院 药学系, 安徽 蚌埠 233030;
    2. 合肥市第四人民医院 药剂科, 安徽 合肥 230022;
    3. 蚌埠医学院第一附属医院 心血管科, 安徽 蚌埠 233004;
    4. 阜阳市第一人民医院心血管科, 安徽 阜阳 236000
  • 收稿日期:2015-05-11 修回日期:2015-11-27 出版日期:2016-03-28 发布日期:2018-06-12
  • 通讯作者: 唐碧,Tel:15855798487;E-mail:bitang2000@163.com E-mail:bitang2000@163.com
  • 基金资助:
    国家自然科学基金资助项目(81170046);教育部留学回国人员科研启动基金(第46批);安徽省自然科学基金资助项目(10040606Q44)

Effects of docosahexaenoic acid on atrial physiological parameter and TASK-1 protein expression in atrial fibrillation rats

GE Min1, SHAN Feng1,2, TANG Bi3, HOU Xiu-Jie4   

  1. 1. Department of Pharmacy, Bengbu Medical College, Bengbu 233004;
    2. Department of Pharmacy, the Fourth People's Hospital of Hefei, Hefei 230022;
    3. Department of Cardiovascular Medcine, the First Affiliated Hospital of Bengbu Medical College, Bengbu 233004;
    4. Department of Cardiovascular Medcine, the First People's Hospital of Fuyang, Fuyang 236000, China
  • Received:2015-05-11 Revised:2015-11-27 Online:2016-03-28 Published:2018-06-12
  • Supported by:
    国家自然科学基金资助项目(81170046);教育部留学回国人员科研启动基金(第46批);安徽省自然科学基金资助项目(10040606Q44)

摘要: 目的:探讨二十二碳六烯酸(DHA)对大鼠心房颤动(AF)模型心房肌生理特性的影响及相关机制研究。方法:80只乙酰胆碱-氯化钙混合液敏感的SD大鼠分为对照组(CTL组)、DHA处理组(DHA组)、房颤组(AF组)和房颤+DHA处理组(DHA+AF组),观察房颤持续时间;采用全细胞膜片钳技术记录大鼠心房肌细胞动作电位时程(APD)和双孔钾通道TASK-1电流,Western blot测定大鼠心房组织TASK-1蛋白表达。结果:大鼠尾静脉注射乙酰胆碱-氯化钙混合液后,房颤持续时间随实验天数增加而逐渐延长,DHA干预缩短房颤持续时间。与CTL组相比,AF组大鼠心房肌细胞复极50%时的动作电位时程(APD50)和复极90%时的动作电位时程(APD90)明显缩短,心房肌细胞TASK-1电流密度升高,蛋白表达升高(P<0.05)。与AF组相比,DHA+AF组大鼠心房肌细胞APD50和APD90明显延长,TASK-1电流密度和蛋白表达降低(P<0.05)。结论:DHA具有延长房颤大鼠心房肌细胞APD的作用,可能与其下调心房肌TASK-1蛋白的表达从而降低心房肌细胞TASK-1电流密度有关。

关键词: 心房颤动, 二十二碳六烯酸, 心房电重构, TASK-1, 大鼠

Abstract: Objective: To investigate the effect of docosahexaenoic acid (DHA) on atrial physiological parameter and its related mechanisms in atrial fibrillation rats. Methods: Eighty Sprague-Dawley (SD) rats which were sensitive to acetylcholine-calcium chloride mixture were randomly divided into four groups:control (CTL), control treated with DHA (DHA), atrial fibrillation (AF) and atrial fibrillation treated with DHA (DHA+AF). The duration of atrial fibrillation was measured. The atrial myocyte action potential duration (APD) and TWIK-related acid-sensitive K+ channels-1 (TASK-1) currents were recorded by whole-cell patch-clamp technique. The expression of TASK-1 at protein level in atrial tissue was detected by Western blot method. Results: Atrial fibrillation of the rats was induced by acetylcholine-calcium chloride mixture, and the duration time of atrial fibrillation was increased with the drug-induced time prolonged. Compared with control group, the time of atrial myocyte action potential duration at 50% repolarization (APD50) and at 90% repolarization (APD90) were significantly shorten in AF group, TASK-1 current density and TASK-1 protein expression were increased (P<0.05). Compared with AF group, the duration of atrial fibrillation was decreased,the time of atrial myocyte APD50 and APD90 were prolonged, TASK-1 current density and protein expression were significantly reduced in DHA+AF group (P<0.05). Conclusion: DHA can prolong the atrial myocyte APD in atrial fibrillation rats, which may be related to down-regulation of TASK-1 protein expression and decreasing TASK-1 current density in atrial tissue.

Key words: atrial fibrillation, docosahexaenoic acid, atrial electrical remodeling, TASK-1

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