5-HTR2B、E-cad、α-SMA在博莱霉素致大鼠肺纤维化中的表达变化

doi:10.13459/j.cnki.cjap.2016.04.020

中国应用生理学杂志 ›› 2016, Vol. 32 ›› Issue (4): 365-369.doi: 10.13459/j.cnki.cjap.2016.04.020

5-HTR2B、E-cad、α-SMA在博莱霉素致大鼠肺纤维化中的表达变化

涂容芳, 张秀峰, 何振华

南华大学附属第二医院呼吸内科, 湖南衡阳 421001

收稿日期:2015-06-04 修回日期:2016-03-04 出版日期:2016-07-28 发布日期:2018-06-20
通讯作者: 何振华,Tel:13974755267;E-mail:1425271618@qq.com E-mail:1425271618@qq.com
基金资助:
何振华,Tel:13974755267;E-mail:1425271618@qq.com

The expression of 5-HTR2B、E-cad、α-SMA in the pulmonary fibrosis in rats

TU Rong-fang, ZHANG Xiu-feng, HE Zhen-hua

Department of Respiratory Medicine, the Second Affiliated Hospital of University of South China, Hengyang 421001, China

Received:2015-06-04 Revised:2016-03-04 Online:2016-07-28 Published:2018-06-20
Contact: 何振华,Tel:13974755267;E-mail:1425271618@qq.com E-mail:1425271618@qq.com
Supported by:
何振华,Tel:13974755267;E-mail:1425271618@qq.com

摘要/Abstract

摘要： 目的：观察5-羟色胺2B受体（5-HTR2B）、E-钙粘素（E-cad）、α-平滑肌蛋白（α-SMA）在博莱霉素（BLM）致大鼠肺纤维化中的表达变化。方法：健康雄性SD大鼠45只，随机分为3组（n=15）：对照组、BLM组、泼尼松+BLM组。各组分别于第7、14和28天各处死动物5只，取肺组织，行HE、Masson染色，观察大鼠肺组织炎症和纤维化的程度；免疫组化法及逆转录-聚合酶链反应（RT-PCR）法检测肺组织中5-HTR2B、上皮细胞标记物E-cad和间质细胞标记物（α-SMA） mRNA及蛋白的表达水平。结果：BLM组肺组织病理切片HE和Masson染色按时间顺序呈现由肺泡炎症至纤维化的动态改变。免疫组化及RT-PCR结果显示肺纤维化大鼠肺组织中5-HTR2B、α-SMA的蛋白及mR-NA表达均增加（P<0.05），28 d时最高；E-cad蛋白及mRNA表达均减弱（P<0.05），28 d时最低；泼尼松+BLM组，相应时间点5-HTR2B、α-SMA蛋白及mRNA表达有所下降（P<0.05），E-cad蛋白及mRNA表达不同程度增强（P<0.05）。结论：5-HTR2B可促进大鼠肺纤维化发生，机制可能与减弱E-cad，促进α-SMA表达，诱导上皮细胞间质转化（EMT）有关。

关键词: 5-HT2B受体, 肺纤维化, 上皮细胞间质转化, 博莱霉素, 大鼠

Abstract: Objective:To observe the expression of 5-Hydroxytryptamine 2B receptor (5-HTR2B)、E-cadherin (E-cad)、alpha-smooth muscle actin(α-SMA) in the bleomycin -induced pulmonary fibrosis tissue of rats. Methods:Forty-five healthy male SD rats were randomly di-vided into control group,bleomycin group and bleomycin+prednisone group(n=15). Five rats in each group randomly sacrificed at the 7^th、the 14^th and the 28^th day after eastablishing models. The lung tissue was observed by microscope in HE and Masson staining. Lung hydroxypro-line (HYP) content was evaluated. The expression of protein and mRNA of 5-HTR2B, E-cad and α-SMA were analyzed by immunohistoche-mistry and/or RT-PCR. Results:A dynamic changes from alveolitis to pulmonary fibrosis could be observed in the slices by HE and Masson staining. The experimental results by immunohistochemistry and RT-PCR showed that the protein and mRNA expression of 5-HTR2B and a-SMA enhanced rat pulmonary fibrosis (P<0.05) and reached the highest at the 28^th day; the corresponding protein and mRNA expression of E-cad reduced (P<0.05), and reduced to the lowest value at the 28^th day. Compared with bleomycin group, the corresponding mRNA and protein expression of 5-HTR2B and a-SMA in bleomycin+prednisone group had decreased (P<0.05); however, the corresponding mRNA and protein expression of E-cad had increased(P<0.05). Conclusion:5-HTR2B is involved in the pathogenesis of pulmonary fibrosis throught epithelial-mesenchymal transition (EMT).

Key words: 5-HTR2B, pulmonary fibrosis, epithelial-mesenchymal transition, bleomycin, rat

涂容芳, 张秀峰, 何振华. 5-HTR2B、E-cad、α-SMA在博莱霉素致大鼠肺纤维化中的表达变化[J]. 中国应用生理学杂志, 2016, 32(4): 365-369.

TU Rong-fang, ZHANG Xiu-feng, HE Zhen-hua. The expression of 5-HTR2B、E-cad、α-SMA in the pulmonary fibrosis in rats[J]. CJAP, 2016, 32(4): 365-369.

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5-HTR2B、E-cad、α-SMA在博莱霉素致大鼠肺纤维化中的表达变化

The expression of 5-HTR2B、E-cad、α-SMA in the pulmonary fibrosis in rats

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