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CJAP ›› 2018, Vol. 34 ›› Issue (3): 249-253.doi: 10.12047/j.cjap.5615.2018.059

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Effects of calcitonin gene-related peptide on epithelial-mesenchymal transition of alveolar epithelial cells in rat and its mechanism

LI Xian-wei1, JIANG Li-li1, ZUO Dong-ze1, HAO Wei1, WU Hai-long2   

  1. 1. Department of Pharmacology, Wannan Medical College, Anhui Provincial Engineering Technology Research Center for Polysaccharide Drugs, Wuhu 241002;
    2. College of Life Sciences, Anhui Normal University, Wuhu 241000, China
  • Received:2017-07-10 Revised:2017-11-20 Online:2018-05-28 Published:2018-09-08
  • Supported by:
    安徽省高校优秀青年人才支持计划重点项目(gxyqZD2016170)

Abstract: Objective: To observe the effects of calcitonin gene-related peptide (CGRP) on epithelial-mesenchymal transition (EMT) of alveolar epithelial cells and its mechanism.Methods: The RLE-6TN cell were divided into 6 groups as follows:control group, cells were incubated with double distilled water (TGF-β1 solvent) for 48 h; transforming growth factor-β1(TGF-β1) group, cells were incubated with TGF-β1 (5 ng/ml) for 48 h; +CGRP (1, 10,100 nmol/L) group, cells were pre-treated with CGRP (1, 10,100 nmol/L) for 1 h, and then subjected to TGF-β1 for 48 h; CGRP 100 nmol/L + CGRP8-37 1 μmol/L group, cells were pre-treated with CGRP 100 nmol/L + CGRP8-37 1 μmol/L for 1 h, and then subjected to TGF-β1 for 48 h. RLE-6TN cell vitality was determined by MTT. The mRNA or protein expression levels of Notch1, eIF3a, α-SMA, E-cadherin and collagen Ⅲ were detected by real-time PCR or Western blot.Results: Compared with the TGF-β1 group, cell vitality and the expression of E-cadherin were significantly increased, and the expression of Notch 1, eIF3a, α-SMA and collagen Ⅲ were markedly decreased in CGRP (1, 10, 100 nmol/L) group. The effects of CGRP above mentioned could be abolished by CGRP 8-37.Conclusion: These results suggest that CGRP inhibits TGF-β1-induced EMT process, and down-regulation of the expressions of Notch1and eIF3a may be involved in its mechanisms.

Key words: calcitonin gene-related peptide, epithelial-mesenchymal transition, Notch1, eIF3a, rat

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